Cynthia Brame

Assistant Professor, Department of Biology

Contact Information

254 Leo Avenue
Shreveport, LA 71105
Home Phone: (318) 865-5058
Work Phone: (318) 869-5097
Email:

Education

• Ph.D. in Pharmacology, Vanderbilt University, 1994-1999
  Dissertation: Discovery of isolevuglandins, novel ketoaldehyde lipid peroxidation products, and characterization of their protein adducts.
• B.S., magna cum laude in Biochemistry and Molecular Biology, Centre College
  Minor in Chemistry

Professional Experience

• Assistant professor, Sept. 2003-present
  Department of Biology
  Centenary College, Shreveport, LA

• Assistant professor, gratis appointment, May 2006-present
  Department of Biochemistry
  LSU-Health Science Center, Shreveport, LA

• Assistant professor, gratis appointment, Sept. 2004-present
  Department of Pharmacology and Experimental Therapeutics
  LSU-Health Science Center, Shreveport, LA

• Assistant professor (one-year appointment), Sept. 2002-Aug. 2003
  Department of Biology
  Mary Baldwin College, Staunton, VA

• Research scientist, Jan. 2001-Aug. 2002
  MDS-Proteomics, Charlottesville, VA

• Post-doctoral Fellow with Professor Donald F. Hunt, Sept. 1999-Dec. 2000
  University of Virginia, Charlottesville, VA

• Graduate student research assistant to Professor L. Jackson Roberts, II, 1994-1999
  Vanderbilt University, Nashville, TN

Publications

Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF, and Horwitz, AR. Paxillin phosphorylation sites mapped by mass spectrometry. J. Cell Science 118: 4925-4929, 2005.

Moran MF, White F, Marto J, Brame CJ, Ornatsky O, Ross M, Toledo-Sherman LM, Castro A, Duewel H, Hosfield C, Orsi C, Topaloglou T, Figeys D, Caldwell-Busby J, and Stover DR. Phospho-proteomics in drug discovery and development. Chapter in: Protein Tyrosine Kinase Inhibitors in Cancer Therapy. D. Fabbro and F. McCormick, eds. Humana Press, 2004.

Marto JA, Brame CJ, Ficarro SB, White FM, Shabanowitz J, and Hunt DF. Sequence analysis: Low energy MS/MS-Peptide Interpretation. Chapter in: Encyclopedia of Mass Spectrometry, Volume 2: Biological Applications. R. Caprioli and M. Gross, eds. Elsevier, 2004.

Marto JA, Brame CJ, Ficarro SB, White FM, Shabanowitz J, and Hunt DF. Chemical derivitization for peptide sequence analysis and consideration for sequence analysis of peptides subjected to posttranslational modifications. Chapter in: Encyclopedia of Mass Spectrometry, Volume 2: Biological Applications. R. Caprioli and M. Gross, eds. Elsevier, 2004.

Brame CJ, Moran MF, and McBroom-Cerajewski, LD. A mass spectrometry-based method for distinguishing between symmetrically and asymmetrically dimethylated arginine residues. Rapid Communications in Mass Spectrom. 18: 877-81, 2004.

Brame CJ, Boutaud O, Davies S, Yang T, Oates JA, Salomon RG, Roden DM, Morrow JD, and Roberts LJ, II. Modification of proteins by isoketal-containing oxidized phospholipids. J. Biol. Chem. 279: 13447-51, 2004.

Zarling AL, Luckey CJ, Marto JA, White FM, Brame CJ, Evans AM, Lehner PJ, Cresswell P, Shabanowitz J, Hunt DF, and Engelhard VH. Tapasin is a facilitator, not an editor, of class I MHC peptide binding. J Immunol. 171: 5287-95, 2003.

Davies SS, Brame CJ, Boutaud O, and Roberts LJ, II. Measurement of isoketal protein adducts by liquid chromatography electrospray tandem mass spectrometry. Chapter in: Methods in Biological Oxidative Stress. K. Hensley and RA Floyd, eds., pp 127-136, 2003.

Harnpicharnchai P, Jakovljevid J, Horsey E, Miles T, Roman J, Rout M, Meagher D, Imai B, Guo Y, Brame CJ, Shabanowitz J, Hunt DF, and Woolford JL, Jr. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell 8: 505-515, 2001.

Boutaud O, Li J, Chaurand P, Brame CJ, Marnett LJ, Roberts LJ, II, and Oates JA. Oxygenation of arachidonic acid by cyclooxygenases generates reactive intermediates that form adducts with proteins. Adv Exp Med Biol. 500:133-137, 2001.

Strahl BD, Briggs SD, Brame CJ, Caldwell JA, Koh SS, Ma H, Cook RG, Shabanowitz J, Hunt DF, Stallcup MR, and Allis CD. Methylation of histone H4 at arginine 3 occurs in vivo and is mediated by the nuclear receptor coactivator PRMT1. Curr. Biol. 11: 996-1000, 2001.

Boutaud O, Brame CJ, Chaurand P, Li J, Rowlinson SW, Crews BC, Ji C Marnett LJ, Caprioli RM, Roberts LJ, II, and Oates JA. Characterization of the lysyl adducts of prostaglandin H-synthases that are derived from oxygenation of arachidonic acid. Biochemistry 40: 6948-6955, 2001.

Mosammaparast N, Jackson KR, Guo Y, Brame CJ, Shabanowitz J, Hunt DF, and Pemberton LF. Nuclear import of histone H2A and H2B is mediated by a network of karyopherins, including Kap114p, Kap121p, Kap123p, and Kap95p. J. Cell. Biol. 153: 251-262, 2001.

Roberts LJ, II, Chen Y, Boutaud O, Davies SS, Morrow JD, Oates JA, and Brame CJ. Reactive products of the isoprostane pathway: isoketals and cyclopentenone A2/J2-isoprostanes. Chapter in: Advances in Prostaglanding and Leukotriene Research: Basic Science and New Clinical Applications. B. Samuelsson, R. Paoletti, GC Folco, E. Granstrom, and S. Nicosia, eds. Kluwer Academic Publishers, 2001.

Hsu J-Y, Sun Z-W, Li X, Reuben M, Tatchell K, Biship DK, Grushcow JM, Brame CJ, Caldwell JA, Hunt DF, Lin R, Smith MM, and Allis CD. Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell 102: 279-291, 2000.

Reich EE, Zackert WE, Brame CJ, Chen Y, Roberts LJ, II, Hachey DL, Montine TJ, and Morrow JD. Formation of novel D-ring and E-ring isoprostane-like compounds (D-4/E-4-neuroprostanes) in vivo from docosahexaenoic acid. Biochemistry 29: 2376-2383, 2000.

Roberts LJ, II, Brame CJ, Chen Y, and Morrow JD. Formation of novel reactive products via the isoprostane pathway. In: Proc. 5th Internat. Conf. On Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Other Related Diseases. KV Honn, S Nigam, L Marnett, and E Dennis, eds., Plenum Publishers, 2000.

Salomon RG, Sha W, Brame CJ, Kaur K, Subbanagounder G, O’Neil J, Hoff HF, and Roberts LJ, II. Protein adducts of iso4levuglandin E2 in oxidized low density lipoprotein. J. Biol. Chem. 274: 20271-20280, 1999.

Brame CJ, Boutaud O, Oates JA, and Roberts LJ, II. Characterization of lysyl adducts formed by the -ketoaldehyde prostanoids LGE2/D2. Biochemistry 38: 9389-9396, 1999.

Roberts LJ, II, Salomon RG, Morrow JD, and Brame CJ. New developments in the isoprostane pathway: identification of novel highly reactive -ketoaldehydes (isolevuglandins) and characterization of their protein adducts. FASEB J. 13: 1157-1168, 1999.

Roberts LJ, II, Brame CJ, Chen Y, Morrow JD, and Salomon RG. Formation of reactive products of the isoprostane pathway: isolevuglandins and cyclopenteneone isoprostanes. Adv. Exp. Med. & Biol. 469: 335-341, 1999.

Brame CJ, Salomon RG, Morrow JD, and Roberts LJ, II. Identification of extremely reactive -ketoaldehydes as products of the isoprostane pathway. J. Biol. Chem. 274: 13139-13146, 1999.

Roberts LJ, II, Brame CJ, Chen Y, and Morrow JD. Novel eicosanoids: Isoprostanes and related compounds. Methods Mol. Biol. 120: 257-285, 1999.

Morrow JD, Chen Y, Brame CJ, Yang J, Sanchez SC, Xu J, Zackert WE, Awad JA, and Roberts LJ, II. The isoprostanes: unique prostaglandin-like products of free radical-catalyzed lipid peroxidation. Drug Metab. Rev. 31, 117-139, 1999.

Subbanagounder G, Salomon RG, Murthi KK, Brame CJ, and Roberts LJ, II. Total synthesis of iso4-levuglandin E2. J. Org. Chem. 62: 7658-7666, 1997.

Invited Oral Presentations

• Using phosphoproteomics to understand cell migration. Presented to the November meeting of the local chapter of American Chemical Society, Shreveport, LA. November 2004.

• Using phosphoproteomics to understand cell migration. Presented at the Natural Sciences Division Seminar Series, Centenary College, Shreveport, LA. October 2004.

• Assessing differences between cell populations: Identification of differentially expressed and modified proteins. Presented at the Department of Pharmacology and Therapeutics seminar series, LSU-Health Sciences Center, Shreveport, LA. May 2004.

• Differential expression and phosphorylation of membrane proteins using “gel free” proteomics. Presented at the FASEB Summer Research Conference, Snow Mass, CO. 2001.

• Proteomics: From seeing differences to understanding pathways. Presented at the National Health and Environmental Effects Research Lab of the EPA, Research Triangle Park, NC. 2001.

• Proteomics: an overview of current approaches. Presented at Taylor Technology, Princeton, NJ. 2000.

• Isolevuglandins, highly reactive products of lipid peroxidation, preferentially form phospholipid-esterified adducts with membrane proteins in vivo. Presented at Department of Anesthesiology at the University of Alabama at Birmingham, Birmingham, AL. 1999.

• Isolevuglandins, highly reactive products of lipid peroxidation, rapidly form protein adducts in vivo. Department of Biochemistry and Molecular Biology, Centre College, Danville, KY. 1998.

Student Presentations of Collaborative Research

• Jillian A. Kennedy, Elizabeth M. Wilson, Lucy C. Robinson, and Cynthia J. Brame. The Effect of Deletion of Four Consecutive Histidines on Yeast Casein Kinase 2 Function. Poster presented at Student Research Forum, April 21, 2006.

• Talicia Johnson, Cheynita Metoyer, Lucy C. Robinson, and Cynthia J. Brame. Does instability of a mutant Yck2 protein require Yck2 kinase function? The effect of a kinase domain mutation in an unstable Yck2 mutant. Poster presented at Student Research Forum, April 21, 2006.

• Kelly Waterhouse, Julia Reynolds, Lucy C. Robinson, and Cynthia J. Brame. Does deletion of a large glutamine/alanine-rich sequence alter function or localization of yeast casein kinase 2? Poster presented at Student Research Forum, April 21, 2006. Awarded second place in poster presentations.

• Brittany Bjerke, Lucy C. Robinsin, and Cynthia J. Brame. The effects of the conversion of a lysine to an arginine in the catalytic site of Yck2 in Saccaromyces cerevisiae. Oral presentation at Student Research Forum, April 21, 2006.

• Travis Reece, Michelle McNamara, Allyson Sandifer, Lucy C. Robinson, and Cynthia J. Brame. Membrane localization of Yeast Casein Kinase 2 by palmitoylation: Does distance from the carboxyl terminus matter? Oral presentation at Student Research Forum, April 21, 2006.

• Brett Martin, Courtney Rome, Jennifer Wilson, Ashley Williams, Lucy C. Robinson, and Cynthia J. Brame. Effects of the deletion of 17 amino acids from the carboxyl terminus of Yeast Casein Kinase 2. Oral presentation at Student Research Forum, April 21, 2006. Awarded first place in oral presentations.

• Beth Allen, Lucy C. Robinson, and Cynthia J. Brame. The effect of Yck phosphorylation of SEC4 on vesicle function with the plasma membrane. Poster presentation at Student Research Forum, April 2005.

Honors, Awards, and Grants

• Centenary Research Professorship in Natural Sciences, 2006-2008. Student investigations of Yck2 structure and function via mutagenesis followed by analysis of function and localization. $3000/year for two years.
• Louisiana Board of Regents Support Fund Educational Enhancement Grant for “Enhancement of Biomolecular Tools at Centenary College” in collaboration with Dr. Scott Chirhart. Awarded April 2006. $47,802 for one year.
• Louisiana Board of Regents Support Fund Educational Enhancement Grant for “Proteomics in the Undergraduate Curriculum. Awarded April 2006. $47,803 for one year.
• Zeta Tau Alpha January Professor of the Month. Awarded January 2006.
• National Science Foundation Grant for “Identification of substrates for the yeast Yck2 protein kinase” in collaboration with Dr. Lucy Robinson. Awarded September 2005. $370,838 for three years.
• Louisiana Board of Regents Grant within Research and Development Program and Research Competitiveness Subprogram for “Identification of substrates for the yeast Yck2 protein kinase” in collaboration with Dr. Lucy Robinson. Awarded June, 2005. $66,920 for two years.
• Faculty Summer Research Grant, Summer 2005.
• Patent granted: Methods of Detecting Protein Arginine Methytransferase, and Uses Related Thereto. United States Patent 6,747,273, granted June 8, 2004.
• Mattie Allen Broyles Inaugural Year Research Chair, Sept. 2003-Sept. 2004.
• Young Investigator Award. Oxygen ’98 and ’97, 5th and 4th annual meetings of the Oxygen Society.
• Dissertation Enhancement Award. Grant awarded by the Graduate School for expansion of thesis work, 1998.
• Predoctoral Fellowship, Pharmaceutical Research and Manufacturers of America (PhRMA) Foundation, 1997-1998.
• Vanderbilt University Graduate School Fellowship, 1994-1998.
• Interdisciplinary Graduate Program Fellowship, 1994-1995.
• Phi Beta Kappa, academic honor society, 1994.
• Omicron Delta Kappa, honor society for leadership, 1994.
• Barry Goldwater Scholarship for potential in science research, 1993-1994.